Nucleic Acid Labeling and Detection
Molecular Devices dsDNA Assay Kit, Explorer, 5 pg-3 ng
The SpectraMax Quant AccuBlue Pico dsDNA Assay Kit is a fluorescence-based assay for sensitive quantitation of double-stranded DNA in low-concentration samples. The Explorer kit provides reagents sufficient for two 96-well microplates and is optimized for use with microplate readers.
- Sensitive fluorescence-based dsDNA quantitation from 5 pg to 3 ng.
- Explorer kit format sufficient for two 96-well microplates.
- Includes dsDNA-selective fluorescent dye and calibration standards.
- Optimized for low-volume microplate assays and microplate readers.
Cayman Chemical PuromycIn AmInonucleosIde 25mg
The aminonucleoside portion of PANS that is used to induce nephropathy in laboratory animal models to study focal and segmental glomerulosclerosis, the induction of nephrosis, and the events leading to proteinuria in rats
Abcam Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)
Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical O-Propargyl-puromycIn 1mg
A clickable form of puromycin; inhibits protein synthesis in rabbit reticulocyte lysates and HEK293T cells from 1-25 µM; incorporated into nascent polypeptide chains where it forms covalent adducts and terminates chain elongation on the ribosome; has been used in combination with fluorescent azides to image nascent proteins in various cells
ACROBiosystems RNase Activity Assay Kit (Fluorescence)
RNases are a class of hydrolytic enzymes that catalyzes both the in vivo and in vitro degradation of ribonucleic acid (RNA) molecules into smaller components. RNase enzymes are categorized into two groups: exoribonucleases and endoribonucleases. RNases are ubiquitous in the environment, and in some biological materials, they are present in relatively high concentrations. RNases also frequently contaminate common molecular biological reagents such as reaction buffers, enzymes such as reverse transcriptase and RNA polymerase, and buffers for RNA purification and storage. It is often used DEPC and/or heaten to remove RNases from containers in experiments. Since even only minute amounts of RNase contamination would ruin the experiment, it is necessary to evaluate the presence of RNase with the RNase Activity Assay Kit (Fluorescence).
New England Biolabs, Inc. WarmStart LAMP Kit (DNA & RNA) – 100 reactions
The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection, and end-point visualization.
New England Biolabs, Inc. HiScribe™ SP6 RNA Synthesis Kit – 50 reactions
The HiScribe SP6 RNA Synthesis Kit is designed for the in vitro transcription of RNA using SP6 RNA Polymerase. This kit is suitable for synthesis of high yield RNA transcripts and for incorporation of cap analogs (not included) or modified nucleotides (not included) to obtain capped, biotin-labeled or dye-labeled RNA. The kit is also capable of synthesizing high specific activity radiolabeled RNA for use as probes or targets. RNA synthesized from this kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, probes for RNase protection or gel shift assays, hybridization-based blots, anti-sense RNA or RNAi experiments, microarray analysis, microinjection, sgRNA synthesis and in vitro translation studies. This kit contains sufficient reagents for 50 reactions of 25 µl each. Each standard reaction yields >= 80 µg of RNA from 1 µg SP6 Control Template DNA. Each kit can yield >= 4 mg of RNA.
ACROBiosystems RNase Activity Assay Kit (Fluorescence)
RNases are a class of hydrolytic enzymes that catalyzes both the in vivo and in vitro degradation of ribonucleic acid (RNA) molecules into smaller components. RNase enzymes are categorized into two groups: exoribonucleases and endoribonucleases. RNases are ubiquitous in the environment, and in some biological materials, they are present in relatively high concentrations. RNases also frequently contaminate common molecular biological reagents such as reaction buffers, enzymes such as reverse transcriptase and RNA polymerase, and buffers for RNA purification and storage. It is often used DEPC and/or heaten to remove RNases from containers in experiments. Since even only minute amounts of RNase contamination would ruin the experiment, it is necessary to evaluate the presence of RNase with the RNase Activity Assay Kit (Fluorescence).
Cayman Chemical albonIger PuromycIn 10mg
An inhibitor of protein synthesis; inhibits protein synthesis by 99% in vitro; an inhibitor of PSA and APN (IC50s = 9.7 and 41 µM, respectively); reduces viability of HL-60 and MOLT-4 cells (EC50s = 0.055 and 0.17 µM, respectively); active against the chloroquine-sensitive and -resistant P. falciparum strains T9-96 and K1 (IC50s = 0.024 and 0.023 µM, respectively); has been used as a selective marker in cell culture systems and has been chemically modified for use in labeling or imaging newly synthesized proteins
Abcam Nuclear Green LCS1
Nuclear Green LCS1
The product is subject to the following: Abcam Restricted Use Statement